By Henry Y. Wang and Tadayuki Imanaka (Eds.)
content material: creation of engineered antibodies in myeloma and hybridoma cells : improvements in gene expression and approach layout / D.K. Robinson, D. DiStefano, S.L. Gould, G. Cuca, T.C. Seamans, D. Benincasa, S. Munshi, C.P. Chan, J. Stafford-Hollis, G.F. Hollis, D. Jain, ok. Ramasubramanyan, G.E. Mark, and M. Siberklang --
Antibody creation in human hybridomas with growth-associated creation kinetics / Hidekazu Sawada and Kazuaki Kitano --
Antibody construction in chinese language hamster ovary cells utilizing an impaired selectable marker / R.S. Barnett, K.L. Limoli, T.B. Huynh, E.A. Ople, and M.E. Reff --
Antibody construction in insect cells / Kathleen N. Potter, Yucheng Li, and J. Donald Capra --
Antibody expression in vegetation / J. K-C. Ma --
Engineering of immunoglobulin Fc and single-chain Fv proteins in Escherichia coli / David Filpula, Michele Rollence, Nina Essig, James Nagle, Aniruddha Achari, and Timothy Lee --
T-cell designated immunofusion proteins from Escherichia coli : reason, layout, and homes / Marc higher and Patricia A. Nolan --
creation and purification of antibody utilizing Bacillus subtilis as an expression host / S.L. Wong, X.C. Wu, L.P. Yang, S.C. Ng, and N. Hudson --
Trichoderma reesei, a promising novel host for antibody creation / Eini Nyyssönen and Sirkka Keränen --
making use of antibody catalysis to natural synthesis / Brian J. Lavey and Kim D. Janda --
robust and particular peroxidase job with an antibody L chain-porphyrin Fe(III) complicated / Tadayuki Imanaka and Masahiro Takagi.
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Extra resources for Antibody Expression and Engineering
Levels of gene expression from cells following random integration of a gene on a plasmid are influenced by the effects of the local genetic environment at the site of chromosomal integration. This phenomenon is referred to as position effects (7 -6). For immunoglobulins, the range of initial expression from clones derived from the same vector is from <25 ng/ml to >5,000 ng/ml (ME Reff, unpublished data). Previously, our laboratory constructed an expression vector (TCAE) which was designed for die tandem expression of immunoglobulin light and heavy chain genes (7).
77, 6841-6845. 13. S. (1983) Construction and testing of mouse-human heteromyelomas for human monoclonal antibody production. Proc. Natl. Acad. , 80, 7308-7312. 14. , Kida, M . , and Tshukamoto, K. (1985) Establishment of hybrodoma secreting human monoclonal antibiodies against tetanus toxin and hepatitis Β virus surface antigen. Biochem. Biophys. Res. , 129, 26-33. 15. , and Tsukamoto, K. (1987) Establishment of hybridoma secreting human monoclonal antibody against hepatitis Β virus surface antigen.
We routinely use virus at passage three for antibody production. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1995. ch004 tissue culture (is) resulting from the insertion of lepidopteran transposons into the fp25 locus (16,17,18). This FP type of plaque can be confused with recombinant virus plaques. If necessary, it is possible to replaque a viral stock to select for infectious, wild-type or recombinant virus. One study reports that the virus is not appreciably affected by multiple freeze-thaw cycles, but is more sensitive to inactivation by light (21).