Aqueous two-phase systems. Methods and protocols by Rajni Hatti-Kaul

By Rajni Hatti-Kaul

A mix of polymers, or one polymer and a salt, in an aqueous medium separates into stages: this phenomenon comes in handy in biotechn- ogy for product separations. Separation of organic molecules and debris in those aqueous two-phase platforms (ATPS) used to be initiated over forty years in the past via P.-Å. Albertsson, and later proved to be of great software in biochemical and phone organic examine. a lift within the software of ATPS was once obvious whilst difficulties of separations in biotechnology techniques have been encountered. Its simplicity, biocompatibility, and amenability to effortless scaleup operations make using ATPS very appealing for large-scale bioseparations. regardless of the benefits ATPS enjoys over different separation suggestions, the applying of two-phase structures has for a very long time been restrained to chose labora- ries. contemporary years have, notwithstanding, proven a pattern during which expanding numbers of researchers hire two-phase partitioning ideas in either easy and utilized learn.

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2. illustrates turbidometric titration where a series of systems (᭹) are prepared and titrated until a one-phase system is formed—the binodal lies just above this point (᭡). 3. illustrates the determination of nodes (᭿) for systems, which is accomplished by preparing a series of systems lying on different tie-lines (------᭹------) and analyzing the concentration of components in the top and bottom phase. 3. Node Determination 1. Prepare a series of systems and analyze the phase composition of the top and bottom phase.

1990) Extraction of proteins from biological raw materials using aqueous PEG/citrate phase systems. Biotechnol. Appl. Biochem. 12, 397–404. 8. Silva, L. H. , Coimbra, J. S. , and Meirelles, A. J. A. (1997) Equilibrium phase behavior of poly(ethyelene glycol) + potassium phosphate + water twophase systems at various pH and temperatures. J. Chem. Eng. Data 42, 398–401. 9. K. (1991) Molecular weight distribution and aqueous two-phase systems. J. Biotechnol. 20, 151–162. 10. Stewart, R. M. and Todd, P.

Instead of a needle, a very fine tip is drawn from a microcapillary over a suitable flame and cut to provide a small Physical Properties of ATPS 2. 3. 4. 5. 6. 7. 8. 008 cm) for small drops. 0 cm are cut to carry or act as the test surfaces. Software: A standard image-analysis package, such as OPTIMAS™ is used to obtain the drop profile. The software necessary to perform the axisymmetric drop shape analysis by numerical integration of the Laplace equation may be obtained commercially through Dr.

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