Basic Lab Procedures in Clinical Bacteriology by J. Vandepitte, K. Engbaek, P. Piot, C.C. Heuck, P. Rohner

By J. Vandepitte, K. Engbaek, P. Piot, C.C. Heuck, P. Rohner

This guide is a pragmatic advisor, to be used by way of laboratory employees in healthiness centres and district hospitals, to the tactics to be in acquiring specimens, setting apart and deciding on micro organism, and assessing their resistance to antibiotics. It covers bacteriological research of blood, cerebrospinal fluid, urine, stool, sputum, pharyngeal and genital specimens, and purulent exudates. specific recognition is given to the necessity for qc of all laboratory approaches. a listing of media and reagents wanted for the isolation and identity of the most typical bacterial pathogens is incorporated, including a sign in their relative significance for the middleman laboratory. This checklist is meant for edition to neighborhood situations.

This moment version has been up to date in lots of components, together with a drastically superior part on stool specimens and a brand new part on serological exams.

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Acta Pathologica et Microbiologica Scandinavica, Section B, 1976, 84:245–251. 35 A URINE Procedure: 1. 25 ml of saline. The suspension should be prepared from colonies growing on MacConkey agar. 2. 5). 1. 25 ml of the medium into each of the required number of sterile tubes. Close the tubes with stoppers. Label the tubes PGUA and indicate the date. 3. Inoculate one tube of the PGUA medium with a dense suspension of the organism to be tested. Incubate the tube for 4 hours at 35 ∞C. The development of a yellow colour indicates the presence of b-glucuronidase (positive result); if no colour change occurs the result is negative.

Coli (EIEC), enteroadhesive E. coli (EAEC), and enteroaggregative E. coli (EAggEC). Four of them are common causes of diarrhoeal disease in developing countries. However, identification of these strains requires serological assays, toxicity assays in cell culture, pathogenicity studies in animals and gene-probe techniques that are beyond the capacity of intermediate-level clinical laboratories. It may be possible to have a presumptive identification of a VTEC strain, as the most frequent VTEC serotype O157:H7 is characterized by being sorbitol-negative.

Coli strain will need additional identification by serotyping with E. coli O157 antiserum. Demonstration of verotoxin production indicates that it is a VTEC strain. Cholera is a typical example of a toxigenic infection. All the symptoms can be attributed to the intestinal fluid loss caused by an enterotoxin released by Vibrio cholerae in the intestine. The stool is voluminous and watery, and contains no inflammatory cells. The main objective of treatment is fluid replacement and antimicrobials have only a secondary role.

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